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KMID : 0359320030430020181
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Korean Journal of Veterinary Research
2003 Volume.43 No. 2 p.181 ~ p.188
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Modification of Radiation Response in Mice by Dongchongxiacao (Paecilomyces japonica)
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Abstract
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Cordyceps has a reputation for its broad biological activities and as a tonic which replenishes vital function in Chinese traditional medicines. As an attempt to obtain fundamental data for the development of new type Cordyceps, the effects of the fruiting bodies of cultivated fungus of Paecilomyces japonica grown on silkworm larvae an radiation induced damages were investigated. We performed this study to determine the effect of Dongchongxiacao (Paecilomyces japonica) on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells and hair follicles of mice irradiated with high and low dose of gamma-radiation. Treatment with Dongchongxiacao showed no significant modifying effects on the jejunal crypt survival and endogenous spleen colony formation. The frequency of radiation induced apoptosis was reduced by pretreatment of Dongchongxiacao (i.p.: 50mg/kg of body weight, at 12 and 36 hours before irradiation, p<0.01). The spontaneous levels of apoptotic cells are 0.082±0.041 in intestinal crypts and 0.231±0.084 per hair follicle section of skin. Pretreatment of Dongchongxiacao was associated with decrease of 26.86% in intestinal crypt and 66.36% in hair follicle decrease in the number of cells with nuclei positively stained for apoptosis compared with the irradiation control group. We demonstrated for the first time that Dongchongxiacao administration could reduce the extent of apoptosis produced by radiation in the hair follicle. The results presented herein that Dongchongxiacao given before irradiation is capable of reducing the severity of cell loss as a result of apoptosis.
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